Human Embryonic Stem Cell Protocols


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Introduction of hESC into nonhuman animals for the sole purpose of determining pluripotency teratoma formation , provided the hESC is not introduced into the brain or gonads. Research that is permissible only after review and approval by the ESCRO and completion of other reviews mandated by current regulations and University policies: Attempted derivation of new hESC lines from donated blastocysts, from in vitro fertilized oocytes, by nuclear transfer, or from other Embryo-Like Entities.

The application to conduct the research shall set forth: the scientific rationale for the need to generate new hESC lines; the basis for the number of blastocysts or oocytes needed; how privacy rights of the donors are respected; and how donor rights to the biomaterials have been resolved. Introduction of hESCs into humans. Research in which the data relating to the donors of the blastocysts, gametes, or somatic cells from which hESCs are derived is not completely deidentified so that the identity of the donors might become known to the investigator.


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Forms of in vitro research in which human totipotent cells or pluripotent stem cells are mixed with pre-implantation human embryos. In no case shall such experiments be allowed to progress for more than 14 days of development in vitro, or past the point of the primitive streak formation, whichever is first. Research and activities that are not permitted at this time: Research involving in vitro culture of any intact human embryo, regardless of derivation method, for longer than 14 days or until formation of the primitive streak begins, whichever occurs first.

Human Embryonic Stem Cell Protocols

Research in which hESCs are introduced into nonhuman primate blastocysts or in which any embryonic stem cells are introduced into human blastocysts. Research in which any products of research involving human totipotent or pluripotent cells are implanted into a human or non-human primate uterus. Breeding of any animal into which hESCs have been introduced at any stage of development. Research Involving iPS cells Investigators who intend to engage in research using iPS cells must secure ESCRO approvals to the extent specified below prior to conducting such research or expending any funds on it.

Derivation of new human iPS lines.

Purely in vitro research that does not involve the generation of human gametes, embryos, or other types of totipotent cells. Introduction of iPS cells into nonhuman animals for the sole purpose of determining pluripotency teratoma formation , provided the cells are not introduced into the brain or gonads Research that is permissible only after review and approval of the Chair of the ESCRO and completion of other reviews mandated by current regulations and University policies.

Culture and Maintenance of Human Embryonic Stem Cells

Purely in vitro research that involves the generation of human gametes. In vivo research that implants human iPS cells into animals into nonhuman animals except as limited below in VI.

Research that is permissible only after review and approval by the ESCRO and completion of other reviews mandated by current regulations and University policies. In vitro research that involves the generation of embryos or other types of totipotent cells. In vivo research that injects human iPS cells into prenatal nonhuman animals or into the brain of postnatal nonhuman animals.

For example: Research involving hESCs where the donor may be identified by the Yale investigator, including cell lines that retain links such as code to identifiable information, is considered to be Human Subjects Research that requires IRB review.

Author information

Research involving hESCs where there is interaction or intervention with a living individual and information about such individual, or tissue from such individual, is obtained, is considered Human Subjects Research that requires IRB review. Investigators who are using or plan to use hESCs are required to provide the following detailed information to the ESCRO on the designated tracking form for all hESC research, regardless of the source of funding: Sources of the hESCs, including the original sources of derived cell lines, Sources of funding for the research work, and Locations, on or off campus, at which the work will be or is being performed.


  • Bibliographic Information.
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  • Virginia Woolf (Women in the Arts (Philadelphia, Pa.).).
  • Towards a Critical Theory of Society: Collected Papers of Herbert Marcuse, Volume 2 (Herbert Marcuse: Collected Papers).
  • Human Embryonic Stem Cell Protocols door Kursad Turksen (Boek) - cooguacusnosess.ga.
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  • Compliance, Monitoring, and Reporting to Ensure that Established Requirements and Procedures are Implemented as Intended Faculty investigators will be expected to participate in periodic reviews by the Yale University Audit Department to assure that all applicable regulations are being followed, and to evaluate the effectiveness of Yale policies and procedures related to the conduct of hESC Research. Last updated: March, RegenMed Digest - industry updates.

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    Review of Human Embryonic Stem Cell Research at Yale

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    Human Embryonic Stem Cell Protocols Human Embryonic Stem Cell Protocols
    Human Embryonic Stem Cell Protocols Human Embryonic Stem Cell Protocols
    Human Embryonic Stem Cell Protocols Human Embryonic Stem Cell Protocols
    Human Embryonic Stem Cell Protocols Human Embryonic Stem Cell Protocols
    Human Embryonic Stem Cell Protocols Human Embryonic Stem Cell Protocols
    Human Embryonic Stem Cell Protocols Human Embryonic Stem Cell Protocols

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